Skip to main content
Industrial Research And Consultancy Centre
Development of constant temperature microdevice
Development of constant temperature microdevice

In several biological applications, it is required to maintain the cells above / below the room temperature implying the need for heating / cooling the microdevice. For instance, in polymerase chain reaction (PCR) a particular DNA sequence is amplified and used in applications such as pathogen detection and hereditary disorder diagnosis. The entire process takes place in 3 steps: denaturation, annealing and extension for which the different stations are required to be maintained at constant temperatures of 95°C, 55°C and 72°C, respectively. When the temperature of the sample is to be raised, one can either supply a uniform heat flux at the boundaries, or maintain the boundaries at a higher temperature. As is well known, for hydrodynamically and thermally fully-developed flow in simple geometries, a uniform heat flux leads to a linear increase in surface temperature, and a constant difference between the bulk fluid and wall temperatures.

Prof. Amit Agrawal

Research Category