FEG-SEM (JSM-7600F) :

• SEI resolution 1.5 nm (1 kV) in GB mode, 1.0 nm (15 kV)
• Magnification 25 to 1,000,000x (printed as a 120mm x 90mm micrograph)
• Accelerating voltage 0.1 to 30 kV
• Beam current 1 pA to 200 nA at 15 kV
• Aperture angle control lens Integrated
• Detector Upper and lower detectors
• Energy Filter New r-filter
• Gentle beam Integrated
• Digital images 1,280 x 960, 2,560 x 1,920, 5,120 x 3,840
• Specimen exchange chamber Single step loading/unloading
• Specimen stage Eucentric, 5-axis motor drive (Type II)
  • X-Y stage: 110 mm x 80 mm
  • Tilt: -5 to +70^o
  • Rotation: 360^o
  • Working Distance: 1.5 mm to 25 mm
• Vacuum system 2 SIPs, magnetic bearing TMP, RP
• Energy efficient design
  • Normal operation: 1.2 kVA
  • Sleep mode: 1 kVA
  • Operation system OFF: 0.76 kVA

Cryo Preparation System (PP3000T) :

• Variable temperature conduction cooled specimen stage (-185^oC to 50^oC)
• Gas-cooled nitrogen cold stage assembly (-192^oC to 50^oC). Temperature stability of > 1^oC
• Cold stage 'parking space' for a second specimen shuttle (allows a second specimen to be prepared in advance)
• Large anti-contaminator (cold trap) plates above and below cold stage
• Robust micrometer‐fed fracturing knife (actively cooled)
• Side-mounted surface knife/probe (actively cooled). A range of scalpel blades can be fitted to suit different specimen requirements
• Automatic sublimation (controlled and viewed on the touch screen)
• Fully automatic, high resolution sputter coater with platinum (Pt) target.
• Sputtering controlled and viewed on the user touch screen
• High purity argon gas canister (1L)
• Large front viewing window (138 x 73mm) plus top and side viewing ports
• Chamberscope viewing port shutter - automatically closes during sputtering to ensure the port window is clear of sputtered material
• Preparation chamber camera (chamberscope) mounted on a side port

FEG-SEM (JSM-7600F) :

• Ultrahigh resolution comparable to the cold cathode FE-SEM.
• In-Lens Thermal FEG.
• Aperture angle control lens automatically optimizes the spot size at both high and low currents for both analysis and imaging.
• Built-in r-filter enabling user selectable mixture of secondary electron and backscattered electron images.
• Gentle Beam mode for top-surface imaging, reduced beam damage and charge suppression.
• Eco design for energy conservation.

Cryo Preparation System (PP3000T) :

• Column mounted preparation chamber ‐ essential for frost‐free transfer and ease of use
• Cold stage temperature down to 190oC, plus comprehensive cold trapping
• Unsurpassed specimen visibility ‐ large front window, top viewing ports, multiple LED chamber lighting
• Cameras in the preparation chamber and SEM ‐ cumbersome binocular not needed
• Independent gas‐cooling of the SEM cold stage and cold trap ‐ temperature range down to ‐192oC (not possible with conduction cooling)
• Pumped storage of cryo preparation device ‐ ensures transfer rod is maintained in a vacuum compatible condition when not in use

The JSM-7600F The Field Emission Gun (FEG) Scanning Electron Microscope (SEM) was installed at the IIT Bombay in October 2012. The FEG-SEM will be used to obtain high resolution images of materials, cells, and tissues at the micron, sub-micron, and nanometer scale, taking advantage of the high resolution of SEM's with FEG electron sources. The FEG-SEM will also be integrated with a serial sectioning device to achieve sequential SEM images of materials following serial sectioning. The combination of devices will allow 3-dimensional analysis of tissue specimens with low-nanometer resolution in the X-Y plane and Z-plane resolution depending on the serial sectioning device.

The Cryo unit (PP3000T) by Quorum is a highly automated, easy to use, column-mounted, gas-cooled cryo preparation system. The PP3000T has all the facilities needed to rapidly freeze and transfer specimens. The cryo preparation chamber is turbomolecular pumped and includes tools for cold fracturing, controlled sublimation and specimen coating. The specimen can then be transferred onto a highly stable SEM cold stage for observation. Cold trapping in the cryo preparation chamber and SEM chamber ensures the whole process is frost-free.

How do I prepare the sample for the CRYO SEM?

CRYO SEM images hydrated liquid samples. Please come directly with the suspension of particles.  No extra preparation is needed.

What should be the concentration of the particles in suspension?

Low concentration suspensions (<1% v/v) usually lead to better results.

Can I image live Bacteria?

Yes. Bacterial sample should be washed with milliQ water atleast 3 times for good results.

What is the procedure to become a Teaching Assistant (TA) for the facility?

You can send an email request to the cryofegsem@iitb.ac.in.  Your name will be added in the waiting list and we will inform you when the training starts.

What is the tenure of a TA?

The tenure is 2 years including the training. The TA spends 8hrs/wk imaging the samples, of which 4 hours are dedicated for samples of other users while the remaining 4 hours may be used to image samples from TA's lab.

Can I request a particular TA  to image my sample?

No. All TAs have undergone the same training and it should not matter who images your sample. If you have any apprehensions about any TA, feel free to bring it to the notice of the convener immediately.

• Nehra, A.;Yarramala, D. S.; Hinge, V. K.; Samanta, K. and Rao, C. P.Differentiating Phosphates by an Mg²⁺ Complex of the Conjugate of Calix[4]arene via the Formation of Ternary Species and Causing Changes in the Aggregation: Spectroscopy, Microscopy, and Computational Modeling. Analytical Chemistry 2015, 87, 9344-9351.
• Bandela, A. K.; Hinge, V. K.; Yarramala, D. S. and Rao, C. P. Versatile, Reversible, and Reusable Gel of a Monocholesteryl Conjugated Calix[4]arene as Functional Material to Store and Release Dyes and Drugs Including Doxorubicin, Curcumin, and Tocopherol. ACS Applied Materials & Interfaces 2015,7 (21), 11555-11566.
• Bandela, A. K.;Bandaru, S. and Rao, C. P.A Fluorescent 1, 3‐Diaminonaphthalimide Conjugate of Calix [4] arene for Sensitive and Selective Detection of Trinitrophenol: Spectroscopy, Microscopy, and Computational Studies, and Its Applicability using Cellulose Strips. Chemistry-A European Journal,2015,21 (38), 13364-13374.
• K. Tababasum and Rao, C. P. “Zn²⁺ and Cu²⁺ induced nanosheets and nanotubes in six different lectins by TEM” RSC Advances 2015, 5, 16828-16836.
• Mummidivarapu, V. V. S.; Bandaru, S.; Yarramala, D. S.; Samanta, K.; Mhatre, D. S. and Rao, C. P. Binding and Ratiometric Dual Ion Recognition of Zn²⁺ and Cu²⁺ by 1,3,5-Tris-  amidoquinoline Conjugate of Calix[6]arene by Spectroscopy and Its Supramolecular Features by Microscopy. Analytical Chemistry 2015, 87 (9), 4988-4995.
• Mummidivarapu, V. V. S.; Yarramala, D. S.; Kondaveeti, K. K. and Rao, C. P. Time- and Concentration-Dependent Reactivity of Cys, Hcy, and GSH on the Diels–Alder-Grafted 1,3,5-Tris Conjugate of Calix[6]arene To Bring Selectivity for Cys: Spectroscopy, Microscopy, and Its Reactivity in Cells. The Journal of Organic Chemistry 2014,79 (21), 10477-10486.
• K. Tababasum and Rao, C. P.Zn²⁺, Cd²⁺ and Cu²⁺ Mediated Formation of Amyloid like Fibrils by the Monomers of β-Sheet Rich Peanut Agglutinin. RSC Advances 2014,4,53044-53054.
• Mummidivarapu, V. V. S.; Hinge, V. K.; Samanta, K.;Yarramala, D. S. and Rao, C. P.SupramolecularComplexation of Biological Phosphates with an Acyclic Triazolium‐Linked Anthracenyl‐1,3‐Diconjugate of Calix[4]Arene: Synthesis, Characterization, Spectroscopy, Microscopy, and Computational Studies. Chemistry-A European Journal,2014, 20 (44), 14378-14386.
• Areti, S.; Yarramala, D. S.; Samanta, K.; Hinge, V. K.; Khedkar, J. and Rao, C. P. Glyco-conjugate as selective switch-on molecule for Hg²⁺ in the presence of albumin proteins, blood serum milieu and on silica gel solid support. RSC Advances 2014,4 (31), 16290-16297.
• A. Nehra, V.K. Hinge, and C.P. Rao,"Phenylene-diimine capped conjugate of lower rim 1, 3-calix[4]arene as molecular receptor for Mg²⁺ via arm conformational changes followed by aggregation and mimicking the species by molecular mechanics" J. Org. Chem., 2014,79, 5763-5770 .
• PhDthesis of V. V. SreenivasuMummidivarapu entitled “Calix[4,6]arene conjugates: Synthesis, Characterization and ion and molecular recognition studies”
• PhDthesis of KhatijaTabbasum entitled “Metallation and self-assembly of proteins and conjugates of calixarenes: Supramolecular nanostructures.”
• PhDthesis of Anil kumarBandelaentitled “Synthesis, Characterization and application potential of conjugates of calix[4]arene.”
• Bioactive Polymersomes Self-Assembled from Amphiphilic PPO-lycoPolypeptides: Synthesis, Characterization, and Dual-Dye Encapsulation Soumen Das, Dharmendar Kumar Sharma, Suman Chakrabarty, Arindam Chowdhury, and Sayam Sen Gupta Langmuir, 2015, 31 (11), pp 3402–3412
• S.K. Rajanna, D. Kumar, M. Vinjamur, M. Mukhopadhyay, Silica Aerogel
  Microparticles from Rice Husk Ash for Drug Delivery, Ind. Eng. Chem. Res.
  54 (2015) 949–956. DOI: 10.1021/ie503867p
• Shashank, B. S., Sharma, S., Sowmya, S., Latha, A. R., Meenu, P. S., and Singh, D. N, "A Geotechnical Engineering Perspective on Bio-mediated Processes", Environmental Earth Sciences (Communicated)
•  Susmita Sharma, Meenu P. S., Asha Latha R, Shashank B. S., and D. N. Singh^b “Characterization of Sediments from the Sewage Disposal Lagoons for Sustainable Development” Advances in Civil Engineering Materials (Communicated)
• 1.K.Tabbasum and C.P.Rao, “Zn2+ and Cu2+ induced nanosheets and nanotubes
  in six different lectins by TEM” RSC Adv., 5(2015), 16828-16836.
• V. V. S. Mummidivarapu, D. S. Yarramala, K. K. Kondaveeti, C. P. Rao, "Time and concentration dependent reactivity of Cys, Hcy and GSH on the Diels Alder grafted conjugate of 1,3,5-tri-calix[6]arene to bring selectivity for Cys: Spectroscopy and microscopy, and its reactivity in
  cells"J. Org. Chem.,79(2014), 10477-10486.
• V.V. Sreenivasu M, V.K. Hinge, K. Samanta, Deepthi S.Y. and C.P.Rao,"Supramolecularcomplexation of biological phosphates with acyclic
  triazolium-linked anthracenyl-1,3-diconjugate of calix[4]arene: Synthesis, Characterization, Spectroscopy, microscopy and computational studies"Chem.A European Journal, 20(2014), 14378-14386
• A. Nehra, V.K. Hinge, and C.P. Rao,"Phenylene-diimine capped conjugate of lower rim 1, 3-calix[4]arene as molecular receptor for Mg2+ via arm conformational changes followed by aggregation and mimicking the species by molecular mechanics" J. Org. Chem., 79 (2014), 5763-5770
•  S. Areti, D. S. Yarramala, K. Samanta, V. K. Hinge, J. K. Khedkar, C. P. Rao, "Glyco-conjugate as selective switch on molecule for Hg2+ in the presence of albumin proteins, blood serum milieu and on silica gel solid support" RSC Adv., 4(2014), 16290-16297.
• IR 820 stabilized multifunctional polycaprolactoneglycol chitosan composite nanoparticles for cancer therapy† Piyush Kumar and Rohit Srivastava
• Nevin Koshy,1 D. N. Singh,2 Bhagwanjee Jha,3 Srinivas Kadali,4 and Jayant Patil5 Characterization of Na and Ca ZeolitesSynthesized by Various Hydrothermal Treatments of Fly Ash  Advances in Civil Engineering Materials
• Journal of The Electrochemical Society, 163 (3) H252-H259 (2016) Electrochemical Study of Bulk and Monolayer Copper in Alkaline Solution Sachin D. Giri and A. Sarkarz
• Kapoor A.*, Barai A.*, Thakur B., Das A., Patwardhan S. R., Monteiro M., Gaikwad S., Bukhari A., Mogha P., Majumder A., De A., Ray P.#, Sen S.#, "Soft drug-resistant ovarian cancer cells migrate via two distinct mechanisms utilizing myosin II-based contractility", BBA Molecular Cell Research (Accepted), (*: contributed equally. #: co-corresponding authors).
• Das A., Monteiro M., Barai A., Kumar S., Sen S., "MMP proteolytic activity regulates cancer invasiveness by modulating integrins", Scientific Reports, 2017.
• Electrochemical Study of Bulk and Monolayer Copperin Alkaline Solution Sachin D. Giri and A. Sarkar, Journal of The Electrochemical Society,163(3) H252-H259 (2016)
• Agnes A.C., Arif M. and D.N. Singh (2017) "Characterization of the mined residues from a bioreactor landfill", Proceedings of 2nd International Symposium on Coupled Phenomena in Environmental Geotechnics, 6-7 Sepetember, Leeds, UK.
• Sachin D. Giri*, A. Sarkar, Sanjay M. Mahajani, A.K. Suresh,, Electrochemical Reduction of CO2 on Copper Oxidized By Electrochemical Methods ECS Transactions, 75 (48) 19-31 (2017), 10.1149/07548.0019ecst ©The Electrochemical Society
• Halkare, P., Punjabi, N., Wangchuk, J., Nair, A., Kondabagil, K. and Mukherji, S. "Bacteria functionalized LSPR based fiber-optic sensor to detect heavy metals like mercury & cadmium in water", submitted to Analyst.
• Sopan M.Phapal,ChandraHas,P.Sunthar, Spontaneous formation of single Component liposomes from a solution, Chemistry and Physics of Lipids 205 (2017) 25–33
• Chandra Has, Sopan M. Phapal, P. Sunthar, Rapid Single-step Synthesis of Liposomes by Stationary Phase Interdiffusion
• Synthesis and Stability of Water-in-Oil Emulsion Using Partially Reduced Graphene Oxide as a Tailored Surfactant  Tanesh D. Gamot,†, Arup Ranjan Bhattacharyya,*,‡ Tam Sridhar,∥ Fiona Beach,# Rico F. Tabor,⊥ and Mainak Majumder*, DOI: 10.1021/acs.langmuir.7b02320    Langmuir 2017, 33, 10311−10321
• Synthesis and characterization of titanium dioxide encapsulated poly (styrene-co-butyl acrylate-co-acrylic acid) nanocomposite latex.          Nitesh Kumar Ray, Venkat Gundabala⁎
• Textural Alterations in Coal Fly Ash due to Alkali Activation Nevin Koshy1 and D. N. Singh, F.ASCE2
• Patel, Y. S. and S. Mehra (2017). "Synergistic Response of Rifampicin with Hydroperoxides on Mycobacterium: A Mechanistic Study." Frontiers in Microbiology 8(2075).
• Triazole-Linked Quinoline Conjugate of Glucopyranose: Selectivity Comparison among Zn2+, Cd2+, and Hg2+ Based on Spectroscopy, Thermodynamics, and Microscopy, and Reversible Sensing of Zn2+ and the Structure of the Complex Using DFT  Sivaiah Areti, Sateesh Bandaru and ChebroluPulla Rao ACS Omega 2016, 1, 626−635
• ­An injectable, self-healing and stress sustainable hydrogel of BSA as a functional biocompatible material for controlled drug delivery in cancer cells
• Aekta Upadhyay, Ravinder Kandi and ChebroluPulla Rao^* (Communicated)
• George E., Barai A., Shirke P., Majumder A., Sen S., "Engineering Interfacial Migration by Collective Tuning of Adhesion Anisotropy and Stiffness", Acta Biomaterialia, 2018. 
• Kapoor A.*, Barai A.*, Thakur B., Das A., Patwardhan S. R., Monteiro M., Gaikwad S., Bukhari A., Mogha P., Majumder A., De A., Ray P.#, Sen S.#, "Soft drug-resistant ovarian cancer cells migrate via two distinct mechanisms utilizing myosin II-based contractility", BBA Molecular Cell Research, February 2018. (*: contributed equally. #: co-corresponding authors).
• Das A., Monteiro M., Barai A., Kumar S., Sen S., "MMP proteolytic activity regulates cancer invasiveness by modulating integrins", Scientific Reports, 2017
• Shashank B. S., Sharma, S., Sowmya S., Asha Latha R., Meenu P. S. and Singh, D.N., “State-of-the-Art on Geotechnical Engineering Perspective on Bio-mediated Processes”, Environmental Earth Sciences, 2016, 75(3), DOI: 10.1007/s12665-015-5071-6.
• Sharma, S., Meenu P. S., Asha Latha R., Shashank B. S. and Singh, D. N., “Characterization of Sediments from the Sewage Disposal Lagoons for Sustainable Development”, Advances in Civil Engineering Materials. ASTM International, 2016, 5(1), 1-23. DOI: http://dx.doi.org/10.1520/ACEM20150010.
• S Das, R Kumar, NN Jha, SK Maji (2017) Controlled exposure of bio-active growth factor in 3D amyloid hydrogel for stem cells differentiation. Advanced Healthcare Materials, 6(18):1700368; DOI:10.1002/adhm.201700368.
• R Kumar, S Das, GM Mohite, SK Rout, S Halder, NN Jha, S Ray, S Mehra, V Agarwal and SK Maji (2018), Cytotoxic oligomers and fibrils trapped in a gel-like state of α-synuclein assemblies. Angewandte Chemie International Edition, 7(19), 5262-5266; DOI:10.1002/ange.201711854.
• S Das, MK Kumawat, S Ranganathan, R Kumar, J Adamcik, P Kadu, R Paadinhaateri, R Srivastava, R Mezzenga and SK Maji (2018), Cell alignment on graphene-amyloid composites. Advanced Materials Interfaces, 1800621; DOI: 10.1002/admi.201800621.
• Patel YS and Mehra S (2017) Synergistic Response of Rifampicin with Hydroperoxides on Mycobacterium: A Mechanistic Study. Front. Microbiol. 8:2075. doi: 10.3389/fmicb.2017.02075
• Cotta, K. B., Padwal, P., Agarwal, S., Bandyopadhyaya, R. and Mehra, S. (201 , Targeting wild-type and drug-resistant mycobacteria in infected macrophages using drug‐coated nanoparticles. J. Chem. Technol. Biotechnol. (Accepted)
• Patkari, M., Kumbhar, C, Nag, A, Mehra, S, Distinct transcriptomic response of S. coelicolor to ciprofloxacin in a nutrient rich environment. (in revision).
• Patel YS and Mehra S (manuscript under preparation) Repurposing Artemisinin as an anti-mycobacterial agent in combination with Rifampicin.
• Suraj Gupta, A Yadav, S Bhartiya, MK Singh, A Miotello, A Sarkar, N Patel, Co oxide nanostructures for electrocatalytic water-oxidation: effects of dimensionality and related properties, Nanoscale 10 (2018), 8806-8819.
• J. Praveen Kumar, Sachin D.Giri, A.Sarkar, Mesoporous NiO with different morphology: Synthesis, characterization and their evaluation for oxygen evolution reaction, 43 (2018) 15639-15649. 
• George E., Barai A., Shirke P., Majumder A., Sen S., "Engineering Interfacial Migration by Collective Tuning of Adhesion Anisotropy and Stiffness", Acta Biomaterialia, 2018. 
• Kapoor A.*, Barai A.*, Thakur B., Das A., Patwardhan S. R., Monteiro M., Gaikwad S., Bukhari A., Mogha P., Majumder A., De A., Ray P.#, Sen S.#, "Soft drug-resistant ovarian cancer cells migrate via two distinct mechanisms utilizing myosin II-based contractility", BBA Molecular Cell Research, February 2018. (*: contributed equally. #: co-corresponding authors).
• Das A., Monteiro M., Barai A., Kumar S., Sen S., "MMP proteolytic activity regulates cancer invasiveness by modulating integrins", Scientific Reports, 2017
• Shashank B. S., Sharma, S., Sowmya S., Asha Latha R., Meenu P. S. and Singh, D.N., “State-of-the-Art on Geotechnical Engineering Perspective on Bio-mediated Processes”, Environmental Earth Sciences, 2016, 75(3), DOI: 10.1007/s12665-015-5071-6.
• Sharma, S., Meenu P. S., Asha Latha R., Shashank B. S. and Singh, D. N., “Characterization of Sediments from the Sewage Disposal Lagoons for Sustainable Development”, Advances in Civil Engineering Materials. ASTM International, 2016, 5(1), 1-23. DOI: http://dx.doi.org/10.1520/ACEM20150010.
• S Das, R Kumar, NN Jha, SK Maji (2017) Controlled exposure of bio-active growth factor in 3D amyloid hydrogel for stem cells differentiation. Advanced Healthcare Materials, 6(18):1700368; DOI:10.1002/adhm.201700368.
• R Kumar, S Das, GM Mohite, SK Rout, S Halder, NN Jha, S Ray, S Mehra, V Agarwal and SK Maji (2018), Cytotoxic oligomers and fibrils trapped in a gel-like state of α-synuclein assemblies. Angewandte Chemie International Edition, 7(19), 5262-5266; DOI:10.1002/ange.201711854.
• S Das, MK Kumawat, S Ranganathan, R Kumar, J Adamcik, P Kadu, R Paadinhaateri, R Srivastava, R Mezzenga and SK Maji (2018), Cell alignment on graphene-amyloid composites. Advanced Materials Interfaces, 1800621; DOI: 10.1002/admi.201800621.
• Patel YS and Mehra S (2017) Synergistic Response of Rifampicin with Hydroperoxides on Mycobacterium: A Mechanistic Study. Front. Microbiol. 8:2075. doi: 10.3389/fmicb.2017.02075
• Cotta, K. B., Padwal, P., Agarwal, S., Bandyopadhyaya, R. and Mehra, S. (201 , Targeting wild-type and drug-resistant mycobacteria in infected macrophages using drug‐coated nanoparticles. J. Chem. Technol. Biotechnol. (Accepted)
• Patkari, M., Kumbhar, C, Nag, A, Mehra, S, Distinct transcriptomic response of S. coelicolor to ciprofloxacin in a nutrient rich environment. (in revision).
• Patel YS and Mehra S (manuscript under preparation) Repurposing Artemisinin as an anti-mycobacterial agent in combination with Rifampicin.
• Suraj Gupta, A Yadav, S Bhartiya, MK Singh, A Miotello, A Sarkar, N Patel, Co oxide nanostructures for electrocatalytic water-oxidation: effects of dimensionality and related properties, Nanoscale 10 (2018), 8806-8819.
• J. Praveen Kumar, Sachin D.Giri, A.Sarkar, Mesoporous NiO with different morphology: Synthesis, characterization and their evaluation for oxygen evolution reaction, 43 (2018) 15639-15649. 
• George, L., Bavya, M. C., Rohan, K. V., & Srivastava, R. (2017). A therapeutic polyelectrolyte–vitamin C nanoparticulate system in polyvinyl alcohol–alginate hydrogel: An approach to treat skin and soft tissue infections caused by Staphylococcus aureus. Colloids and Surfaces B: Biointerfaces, 160, 315-324.
• Chauhan, D. S., Prasad, R., Devrukhkar, J., Selvaraj, K., & Srivastava, R. (2017). Disintegrable NIR light triggered gold nanorods supported liposomal nanohybrids for cancer theranostics. BioconjugateChemistry, 29, 1510-1518.
• Chauhan, D. S., Indulekha, S., Gottipalli, R., Reddy, B. P. K., Chikate, T. R., Gupta, R.,& Srivastava, R. (2017). NIR light-triggered shrinkable thermoresponsive PNVCL nanoshells for cancer theranostics. RSC Advances, 7, 44026-44034.
• Sasidharan, S., Poojari, R., Bahadur, D., &Srivastava, R. (2018). Embelin mediated green synthesis of quasi-spherical and star-shaped plasmonic nanostructures for antibacterial activity, photothermal therapy and computed tomographic imaging. ACS Sustainable Chemistry & Engineering, 6, 10562–10577.
• Pawar, V., Topkar, H., & Srivastava, R. (2018). Chitosan nanoparticles and povidone iodine containing alginate gel for prevention and treatment of orthopedic implant associated infections. International Journal of Biological Macromolecules, 115, 1131-1141.

• 2019:
• ahan I., George E., Saxena N., Sen S., Silver nanoparticle entrapped soft GelMA gels as prospective scaffolds for wound healing, ACS Applied Bio Materials, 2019. 
• Das A., Barai A., Monteiro M., Kumar S., Sen S., Nuclear softening is essential for protease-independent migration, Matrix Biology, 2019. 

• Nuclear softening is essential for protease-independent migration, Matrix Biology, 2019

•  Peter M, Singh A, Mohankumar K, Jeenger R, Joge P, Gatne M, Tayalia P*. Gelatin based matrices as tunable platform to study in vitro and in vivo 3D cell invasion. ACS Appl. Bio Mater. 2019, Accepted. DOI: 10.1021/acsabm.8b00767.

• Cotta, K., Padwal, P., Agarwal, S., Bandopadhyay, R. and Mehra, S., Targeting wild-type and drug-resistant mycobacteria using dual-functional drug-coated nanoparticles, JCTB, 94(3): 768-776, 2019.

• Patel, Y., Mehra, S., “Repurposing Artemisinin as an anti-mycobacterial agent in synergy with Rifampicin,” (under revision, Tuberculosis).

• 2020:

• Adhyapak, P., Srivatsav, A., Mishra, M., Singh, A., Narayan, R., Kapoor, S*. (2020) Dynamical Organization of Compositionally Distinct Inner and Outer Membrane Lipids of Mycobacteria. Biophysical Journal, 118, 1279-1291.

•  Dadhich, R., Mishra, M., Ning, S., Jana, S., Sarpe, VA., Mahato, J., Duan, M*. Kulkarni, SS*, Kapoor, S*. (2020) A virulence-associated lipid with distinct conformational attributes: Impact on lateral organization of host plasma membrane and signaling. ACS Chem. Biol. 15, 3, 740-750.

• S Ray, N Singh, R Kumar, K Patel, S Pandey, D Datta, J Mahato, R Panigrahi, A Navalkar, S Mehra, L Gadhe, D Chatterjee, AS Sawner, S Maiti, S Bhatia, J Gerez, A Chowdhury, A Kumar, R Padinhateeri, R Riek, G Krishnamoorthy and SK Maji, (2020), α‐Synuclein aggregation nucleates through liquid-liquid phase separation. Nature Chemistry , 12:705–716(2020); DOI:10.1038/s41557-020-0465-9

• Inferring layer-by-layer composition in Au-Ag nanoparticles using a combination of X-ray Photoelectron Spectroscopy and Monte Carlo simulations, I Arif, G Agrahari, AK Gautam, A Chatterjee, Surface Science 691, 121503 (2020)

• A Novel α-MoO3/Single-Walled Carbon Nanohorns Composite as High-Performance Anode Material for Fast-Charging Lithium-Ion Battery, SR Sahu, VR Rikka, P Haridoss, A Chatterjee, R Gopalan, R Prakash, Advanced Energy Materials, 2001627 (2020)

• K Sadani, L Muthuraj, P Nag, M Fernandes, K Kondabagil, S Mukherji     A point of use sensor assay for detecting purely viral versus viral-bacterial samples Sensors and Actuators B: Chemical 322, 128562

• P. Halkare, N. Punjabi, J. Wangchuk, A. Nair, K. Kondabagil and S. Mukherji, “Bacteria functionalized gold nanoparticle matrix based fiber-optic sensor for monitoring heavy metal pollution in water”, Sensors and Actuators B: Chemical 281 (2019), 643-651 [doi.org/10.1016/j.snb.2018.10.119].

• Reddy, B. P. K.; Mishra, S. K.; Ravichandran, G.; Chauhan, D. S.;Srivastava, R.; De, A. Preclinical Evaluation of Multi Stimuli Responsive Core-Plasmonic Nanoshell for Phototriggered Tumor Ablation: A Disintegrable Nanohybrid. Applied Materials Today 2020, 20, 100684.

• Bhawana Singh Tomar, A Shahin and Mahesh S Tirumkudulu,  "Cracking in drying films of polymer solutions”, Soft Matter, 2020,16, 3476-3484 

• Jain, Mehak; Seth, Jyoti; Hegde, Lohitha; Sharma, Kamendra P., Unprecedented Self-Assembly in Dilute Aqueous Solution of Polyethyleneimine: Formation of Fibrillar Network; Macromolecules 2020, Just accepted ma-2020-01501t.

• N Singh, K Patel, A Navalkar, P Kadu, D Datta, D Chatterjee, A Shaw, S Jadhav, SK Maji (2020), Amyloid fibril-based hydrogels for high-throughput tumor spheroid modeling. bioRxiv, DOI:10.1101/2020.12.28.424634.

• S Ray, N Singh, R Kumar, K Patel, S Pandey, D Datta, J Mahato, R Panigrahi, A Navalkar, S Mehra, L Gadhe, D Chatterjee, AS Sawner, S Maiti, S Bhatia, J Gerez, A Chowdhury, A Kumar, R Padinhateeri, R Riek, G Krishnamoorthy   and SK Maji, (2020), α‐Synuclein aggregation nucleates through liquid-liquid phase separation. Nature Chemistry, 12:705–716(2020); DOI:10.1038/s41557-020-0465.

• Mehak Jain, Jyoti R. Seth, Lohitha R. Hegde, Kamendra P. Sharma* “Unprecedented Self-Assembly in Dilute Aqueous Solution of Polyethyleneimine: Formation of Fibrillar Network, Macromolecules 2020, 53, 8974−8981 https://pubs.acs.org/doi/pdf/10.1021/acs.macromol.0c01501

• 2021:

• Sushanth Gudlur, Garima Goyal, Arpan Pradhan, James C. S. Ho, Rohit Srivastava, and Bo Liedberg*, Cationic Liposomes Enable Shape Control in Surfactant-Free Synthesis of Biocompatible Gold Nanorods, Chem. Mater. 2021, 33, 12, 4558–4567 https://doi.org/10.1021/acs.chemmater.1c00973

• Pradhan, A.; Mishra, S.; Basu, S. M.; Surolia, A.; Giri, J.; Srivastava, R.; Panda, D. Targeted Nanoformulation of C1 Inhibits the Growth of KB Spheroids and Cancer Stem Cell-Enriched MCF-7 Mammospheres. Colloids and Surfaces B: Biointerfaces 2021, 202, 111702 https://doi.org/10.1016/j.colsurfb.2021.111702

• Barkha Singh, Rohan Bahadur, Misah Rangara, Mayuri N. Gandhi, and Rohit Srivastava*, Influence of Surface States on the Optical and Cellular Property of Thermally Stable Red Emissive Graphitic Carbon Dots,  ACS Appl. Bio Mater. 2021, 4, 5, 4641–4651, https://doi.org/10.1021/acsabm.1c00379

• Mehak Jain, Jyoti R. Seth, Lohitha R. Hegde, Kamendra P. Sharma* “Unprecedented Self-Assembly in Dilute Aqueous Solution of Polyethyleneimine: Formation of Fibrillar Network, Macromolecules 2020, 53, 8974−8981 https://pubs.acs.org/doi/pdf/10.1021/acs.macromol.0c01501

• Patwardhan S*, Mahadik P, Shetty O, Sen S*, "ECM stiffness-tuned exosomes drive breast cancer motility
  through thrombospondin-1", Biomaterials, 2021, 279: 121185.

• Barai A, Mukherjee A, Das A, Saxena N, and Sen S*, " actinin-4 drives invasiveness by regulating
  myosin IIB expression and myosin IIA localization", J. Cell Sci, 2021: jcs.258581.

• George E, Jahan I, Barai A, Ganesan V, and Sen S* (2021), "High ligand density drives extensive
  spreading and motility on soft GelMA gels", Biomed. Mater. 16, 054103.

• NishadKadam A.Sarkar “A rechargeable zinc–air battery with decoupled metal oxidation and oxygen
  reduction reactions” Journal of Power Sources 230375.

• 2022:

• BS Tomar, MS Tirumkudulu*, W Yu, A Berchielli and P Doshi “Osmotic tablet coatings: Drying stress,
  mechanical properties and microstructure”, Int J Pharm, 617, 121611 (2022)

• Magnetic samples can seriously damage the instrument. As per the manufacturer's instruction magnetic samples are not allowed.
• The sample thickness should be less than 3 mm.
• Any query related to the Cryo-FEG-SEM analysis should be emailed to cryofegsem@iitb.ac.in
• At present this facility is available only to the internal IITB users.

We shall accept online registration only through the IRCC webpage. If you need to cancel your slot, send an email immediately to with an explanation.

 Slots will be provided on a first-come-first-served basis.

 USB drives are strictly prohibited for copying data to minimize virus-related issues. You are requested to bring a new blank CD to transfer your data. All data must be transferred within 7 days of imaging. Without exception.

 Users must be present during the entire slot.

Cancellation Email: "This is to inform you that you have cancelled your slot on the Cryo-FEG SEM instrument. You will now have to re-book your slot. If you want to re-schedule, please request your guide to email the Convener (Prof Mahesh Tirumkudulu : mahesh@che.iitb.ac.in) along with a cc to staff (Ms Meena: cryofegsem@iitb.ac.in)."

• Only online registration through the IRCC webpage will be accepted
• If the appointment is given but the user cannot come, a mail should be immediately sent to cryofegsem@iitb.ac.in to cancel his/her slot.
• Bring a new blank CD for copying the images. The USB drives are not allowed due to computer virus problems.
• If possible, please bring one prior Cryo-SEM micrograph of similar sample while coming for analysis.