A spinning disk confocal microscope, when coupled with a sensitive electron-multiplying charge-coupled device (EMCCD) camera, can exceed the sensitivity of a laser scanning confocal microscope system. The fast acquisition time of a spinning disk confocal microscope also allows the collection of three- or four-dimensional data that may not be possible on other systems. For example, it takes seconds rather than minutes to acquire data of a 15-μm Z-stack with individual slices separated by 0.25 μm (68 μm by 68 μm field of view). The acquisition time is dictated by the intensity of the fluorescence signal, which depends on protein concentration laser power, and the camera's sensitivity. Since the immobilization of live animals may not be complete, fast acquisition time also minimizes the probability of sample movement during image acquisition. This in turn allows high-quality reconstruction of all signals in a three-dimensional space. The spinning-disk confocal microscope facility is suitable for capturing fast-changing phenomena, such as imaging swimming bacteria, lipid droplets, etc. Most powerful tools for live cell imaging.