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Industrial Research And Consultancy Centre

Time Resolved Fluorescence Spectroscopy and Microscopy

Description
Time-resolved fluorescence spectroscopy investigates the change in fluorescence over time of a sample when irradiated with UV, visible, or near-IR light. This decay in fluorescence can be measured over a wide time range: from picoseconds to milliseconds and beyond. This fluorescence lifetime spectrofluorometer is compact, benchtop and customizable for any sort of fluorometry, be it fluorescence detection that your research requires.
 
Specifications/Features

Basic TCSPC Guide

  1. Specifications
    1. Excitation Source

1.1.1. Mai Tai Laser

                   The Mai Tai® Ti:Sapphire oscillator is used for a variety of applications ranging from general spectroscopy to multiphoton microscopy. With its leading performance specifications and largest installed base of any laser of its type, the Mai Tai is truly a cutting-edge tool for basic research and biological imaging.

The Mai Tai® SP ultrafast laser is the ultimate short-pulse oscillator with an unprecedented level of automation and long-term environmental stability. Designed for seeding ultrafast amplifiers, it is capable of producing a broad range of output bandwidths: 60 nm to 10 nm, corresponding to near transform-limited pulse widths from <25 fs to 100 fs. The bandwidth is user-adjustable via the graphical user interface (GUI). This unique feature allows for seeding sub-35 fs, 120 fs, and 2 ps amplifier systems using the same seed laser without manual realignment.

  • Unsurpassed stability over ±10ºC operating environment
  • Fully automated for hands-free operation

Output Characteristics

 

Mai Tai HP

 

Pulse Width

<100 fs

Tuning Range4

690-1040 nm

Average Power at 800 nm2

>2.5 W

Beam Roundness20.9-1.1 
Astigmatism2<10% 
Repetition Rate2, 680 MHz ±1 MHz 
Beam Pointing Stability<50 µrad/100 nm 
Noise2, 7<0.15% 
Stability8<±1% 
Spatial Mode2TEMoo, M2 <1.1 
Polarization2>500:1 horizontal 
Beam Divergence2<1.2 mrad 
Beam Diameter (1/e²)2<1.2 mm 
    

Mai Tai Datasheet

Ultrafast Lasers Brochure

Modelocked Mai Tai Laser Brochure

1.1.2. UHG Ultrafast Harmonic Generators

The UHG series is a user-friendly Harmonic generation module capable of second, third, and fourth harmonic generation for ultrafast oscillators and options for pulse selection. The UHG series provides high-efficiency harmonic generation of femtosecond and picosecond oscillators such as the Spectra-Physics’ InSight®, Mai Tai® and Tsunami®.

  • High efficiency conversion
  • Reduced pulse broadening
  •  
 SHGSHG (in THG)THG
Input Wavelength680-1080 nm
Output Wavelength340-540 nm226-360 nm
Conversion Efficiency340%25%10%
Power with Mai Tai HP41000 mW625 mW250 mW
Power with Tsunami XP51080 mW675 mW270 mW

UHG/Harmonic Generation

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Mai Tai LaserUHG Harmonic Generator
  1.  
    1. DeltaFlex TCSPC Lifetime Fluorimeter

                      The DeltaFlex time correlated single photon counting (TCSPC) system is one of Horiba’s Delta series of next generation time correlated single photon counting lifetime instrumentation. The DeltaFlex offers the ultimate in flexibility.

This system is designed for the measurement of luminescence lifetimes ranging over 11 orders of magnitude, without the need to change cables or cards. The heart of the system is the DeltaHub timing electronics, which offers nearly lossless counting and allows for the measurement of lifetimes from 25ps to 1 second.  Comprised of our high repetition rate sources, high speed detectors and ultra-low deadtime electronics, the DeltaFlex enables fast and efficient acquisition of lifetime data.

The new F-Link bus brings simplicity to modularity. Simply add any component to the instrument and the system automatically detects the new addition making it accessible from the software.

  • Measure lifetimes from 25ps with Mai Tai laser source and PPD detection module
  • Up to 1,000 TCSPC measurements per second – ideal for kinetics studies
  • Monochromator wavelength selection  
  • Easy to add accessories with F-Link bus
  • Comprehensive software package
  • Measurement modes
    • Lifetime – measure 50 ps to 1s
    • Kinetic TCSPC – 1 to 10 000 decays measured sequentially in 1ms to 1min per decay
    • Anisotropy – reconvolution analysis to resolve shorter rotational correlation times
    • Time-Resolved Emission Spectra (TRES) – Collect up to 100 wavelength dependent decays
    • Steady-State

DeltaFlex Specefication Sheet

 

Minimum Lifetime50ps
Shortest measurement time1 millisecond
Repetition rates80 MHz (adjustable from the RF in the Harmonic Generation Module)
Prompt FWHM80-100ps
Deadtime10ns
Wavelength selection

 

  • Emission monochromator as standard 200-800nm.
  • 300-1200nm and 400-1600nm versions optional
  • Excitation and 2nd emission monchromators also available

 

  1.  
    1. HPPD Detector

tThe HPPD is Horiba’s latest development in TCSPC detector technology that combines the benefits of conventional PMT design (wide spectral response and large active area) with the advantages of solid state APD technology (good detection efficiency, negligible after-pulsing and exceptional temporal resolution).

  • 5ps lifetime resolution
  • High quantum efficiency (QE)
  • Reduced afterpulsing resulting in better decay residuals
  • Temperature controlled for stability (Peltier)
  • USB interface
  • Fully shielded design

HPPD Detector TechNote

 

Fluorescence Lifetime Imaging Microscopy (FLIM)

Instrument Model: Microtime-200

Features

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  • Complete confocal system with laser combining unit, inverted microscope body, and dual-channel detection unit
  • Turn-key diode lasers for multicolor excitation of 405, 440, 532 and 640 nm
  • Two parallel detection channels using application-optimized detection with SPADs.
  • Time-Correlated-Single Photon Counting (TCSPC) and TTTR mode for investigation of fast dynamics in FCS and FLIM
  • Piezo scanning for 2D- and 3D-lifetime imaging and accurate point positioning
  • Data acquisition, analysis and visualisation software SymPhoTime 64
 
Application
  • Single Molecule Spectroscopy/Detection
  • Fluorescence Lifetime Imaging (FLIM)
  • Fluorescence Correlation Spectroscopy (FCS)
  • Fluorescence Lifetime Correlation Spectroscopy (FLCS)
  • Foerster Resonance Energy Transfer (FRET)
  • Fluorescence Anisotropy (Polarization)
  • Time-Resolved Photoluminescence (TRPL)

 

Faculty in-charge
Contact Email
trfs@iitb.ac.in trfm@iitb.ac.in
Location

 

Contact No:022-2159-6895