Patent
Method for Amplifying Nucleic Acid from a Target
Abstract
This invention provides a method and a device for the single-step disinfection, thermal lysis and isothermal amplification of nucleic acid from the target cell (particularly targeting Mycobacteria). It simplifies the detection process, making it highly efficient and suitable for point-of-care diagnostics by integrating bot lysis and DNA amplification into one step at a consistent temperature of 65°C.
Type of IP
Faculty
Societal Impact
- Improved Healthcare Accessibility: Makes the diagnosis accessible in remote areas.
- Reduced Healthcare Costs: Lowers the costs of diagnostics significantly, allowing for more widespread testing.
- Enhanced Disease Management: Faster diagnosis leads to quicker treatment initiation, potentially reducing transmission rates
Salient technical features and Advantages of the Technology
- Cost Effectiveness: Eliminates the need for expensive equipment like thermocyclers due to the involvement of
- Single-Step Process: Combines thermal lysis and isothermal amplification into a single reaction step
- Constant Temperature: Operates effectively at 65°C utilizing helicase-dependent amplification thus eliminating the need for a thermocycler
- Simplicity: Eliminates the need for multiple steps and manual intervention, reducing potential errors and procedural complexity
- No Washing or Purification Needed: Simplifies the process by working effectively in the presence of cell debris
- Accessibility: Suitable for point-in-care applications, enabling rapid, on-site diagnostic capabilities
- Efficiency: Streamlines the process by removing the need for disinfection, cell washing or DNA purification, maintaining high sensitivity and specificity
Technology readiness level
3
Current Status of Technology
At present a proof-of-concept has been demonstrated in the lab environment with cultured bacterial samples
Relevant Industries
Healthcare and Diagnostics, Biomedical research, Forensic Science, Agriculture
Applications or Domain
- Medical Diagnostics: Particularly useful for rapid tuberculosis testing at the point-of-care, including remote or resource-limited settings.
- Public Health: Enables quick screening in epidemic situations and in regions of high incidence of disease-causing bacterium.
- Research: Facilitates studies on various types of cells by simplifying sample preparation