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Industrial Research And Consultancy Centre

Lattice Light Sheet Microscopy Facility-IoE WCRL

 The Lattice Light Sheet Microscope (LLSM) is capable of imaging biological systems spanning four orders of magnitude in space and time. The system generates an optical lattice to create an ultra-thin light sheet to image biological samples over long periods of time and with very fine resolution. This allows for 4D living cell imaging, where experiments limited to seconds or minutes on other imaging platforms can be extended to hours. LLSM uses ultra-thin sheets of light to image 3D cellular dynamics for hundreds of volumes at dozens of frames per second at diffraction-limited resolution. An extremely sensitive primary objective coupled with a custom- designed illumination system allows optical sectioning using extremely low photon doses for imaging with unprecedented duration.
Make and Model
3i Lattice Light Sheet Microscope
Available mode for use
3D & 4D Imaging

Objectives: 0.71 NA 3.7 mm WD excitation objective, 1.1 NA 2 mm WD detection objective.

Magnification (effective): 62.5 X

Lasers: 488 nm, 560 nm, 642 nm

Cameras: Two Hamamatsu ORCA-Fusion BT sCMOS Camera

Incubation Stage: Temperature controller (22 – 37 o C), CO 2 , and humidity control, 8 ml and 3 ml sample chambers

Filters: 488-640T 560R custom dichroic mirror with FF01 – 446/523/600/677-25 custom made quad filter

  • Semrock FF01 – 698/70
  • Semrock FF01 – 510/42
  • Semrock FF01 – 600/52

Sample Preparation: All samples must be prepared on a 5 mm circular coverslip. See the details later in this document.

Light Sheet Length & Thickness: Light sheet thickness is the axial resolution of a given excitation wavelength (~650 nm for 488 nm laser). Sheet length can be selected between 15 µm to 75 µm.

Data Storage: 60 TB network attached storage

Workstations: One analysis and one acquisition workstation.

Software Packages: Slidebook software (3i) for both analysis and acquisition station with Deconvolution (nearest neighbour, constrained iterative), cell/particle tracking, co-localization packages.

  • Live and fixed cell imaging.


Faculty in-charge
Contact Email

Imaging Facility, Ground Floor, Department of Biosciences & Bioengineering (BSBE), I.I.T. Bombay, Powai, Mumbai – 400076

Contact No: 022-2576-6719

Other Contact Person
  • Dr. Debayan Purkait
Facility Management Members

Prof. Roop Mallik
Prof. Anirban Banerjee
Prof. Shamik Sen
Prof. Arindam Chowdhury
Prof. Nitin Kumar

More Details Here..