Skip to main content
Industrial Research And Consultancy Centre

Laser scanning confocal facility @BSBE

Laser scanning confocal microscopy employs spatial filtering techniques to eliminate any out-of-focus light in specimens with finite thickness and leads to the formation of high-resolution images.
Make and Model
Carl Zeiss, LSM 780
Available mode for use
Single colour and two-colour imaging and brightfield imaging Z-stacks Time series (with or without Z-stack)
  • Travel range of 250 μm.
  • Objectives: Plan-apochromat 10X/0.45 NA(air), 20X/0.8 NA (air), C-apochromat 40x/1.2 NA (water) for FCS measurements, Plan-Apochromat 40x/1.3 NA (oil) iPlan-apochromat 63x/1.4 NA (oil), iPlan-apochromat 100x/1.4 (oil). DIC imaging is possible with the last three objectives.
  • Lasers: Ar+laser, DPSS laser and HeNe laser, Titanium sapphire multiphoton laser
  • Zen 2012 acquisition software
  • Temperature and CO2 -controlled incubation stage for long term live cell imaging.
  • Lifetime imaging through a FLIM attachment
  • Non de-scanned detector available for use with the multiphoton laser for deep tissue imaging.
  • Multi-colour imaging
  • Z- stacks and 3D image reconstruction
  • Time series (with or without Z-stack)
  • Tile scanning to image different parts of the sample automatically over long durations)
  • FLIM


Faculty in-charge
Contact Email

Central Instrumentation Room, Ground floor Biosciences & Bioengineering Department. I.I.T. Bombay, Powai, Mumbai - 400 076.

Contact No: 022-2159 6746

Other Contact Person
  • Pradip Shinde
  • Santosh Panigrahi