Environmental Scanning Electron Microscope Facility

ESEM instrument
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The FEI Quanta 200 scanning electron microscope is a versatile microscope with a tungsten electron source and three modes of operation. High vacuum (HV) mode allows analysis of electrically conducting and non conducting samples (non conducting samples can be analysed after sputter coating) Low Vacuum & ESEM modes allow analyzing biological and insulating samples without coating. This instrument also has EDS facility for chemical characterisation.

Make and Model


Available mode for use

  • High Vacuum mode

    Low Vacuum mode

  • Low vacuum mode

  • Environmental scanning electron microscope mode


  • Resolution: 3.5 nm

  • Accelerating voltage: 0.2kV to 30kV

  • Emission current: 100uA

  • Magnification range: low 20X, High 50,000X


  • Materials Science/Metallurgy
    Fractography (samples which have bigger dimensions can be analysed)
    Micro drilling and cutting tools
    Dental and medical  
    Polymer science
    Energy science/Engg.
    Biological and life sciences
    Civil and Earth Sciences

  • Biological Science

  • Nanotechnology

  • Ceramics

  • Pharmaceuticals

  • Semiconductors

Facility in-charge

Contact Email

esem[at] iitb[dot] ac[dot] in

Contact no.



Room No. 305, Ground Floor,

Sophisticated Analytical Instrument Facility

Centre for Research in Nanotechnology and Science

Other contact person(s)

  • Mrs. Mayura S Chaubal
  • Ms. Rashmi Poojari
  • Mr. Naresh Ambati

Registration Link:

Submit New Request

Technical Specifications

Instrument Details

  1. Make : FEI
  2. Model : QUANTA 200
  3. Specification : Operating voltages : 0.2kV to 30kV Resolution :3.5 nm
Publications using data from this facility


1. Effects of Ethanol Addition on the Size Distribution of Liposome Suspensions in Water, Ankush Pal, P. Sunthar, D. V. Khakhar*, Ind. Eng. Chem. Res. 2019, 58, 18, 7511–7519

2. Efficient separation of biological macromolecular proteins by polyethersulfone hollow fiber ultrafiltration membranes modified with Fe3O4 nanoparticles …, A Modi, J Bellare - International journal of biological macromolecules, 2019 - Elsevier



1. R. Ragipani, S. Bhattacharya, A.K. Suresh, Understanding dissolution characteristics of steel slag for resource recovery, Waste Management, 117(2020), 179-187

2. Siddharam Manea , Suhas S. Joshia, Shyamprasad Karagaddea , Shiv G. Kapoor, Modeling of variable friction and heat partition ratio at the chip-tool interface during orthogonal cutting of Ti-6Al-4V,Journal of manufacturing procresses 55(2020) 254-267

3. The consequence of silicon additive in isothermal decomposition of hydrides LiH, NaH, CaH2 and TiH2 R Kalamkar, V Yakkundi, A Gangal - … Journal of Hydrogen Energy, 2020

4. Synthesis and properties of amino and thiol functionalized graphene oxide, HP Manwatkar, SD Gedam, CS Bhaskar… - Materials Today …, 2020 - Elsevier

5. Impact of thermal annealing inducing oxidation process on the crystalline powder of In 2 S 3, A Timoumi, W Zayoud, A Sharma, M Kraini… - Journal of Materials …, 2020 - Springer

6. Unique Structure-Induced Magnetic and Electrochemical Activity in Nanostructured Transition Metal Tellurates Co1 – xNixTeO4 (x = 0, 0.5, and 1)copyright © 2020 American Chemical Society

7. Amoxicillin removal using polyethersulfone hollow fiber membranes blended with ZIF-L nanoflakes and cGO nanosheets: Improved flux and fouling-resistance, A Modi, J Bellare - Journal of Environmental Chemical Engineering, 2020 - Elsevier

8. La/Ce mixed metal oxide supported MWCNTs as a heterogeneous catalytic system for the synthesis of chromeno pyran derivatives and assessment of green …, RA Rather, S Siddiqui, WA Khan, ZN Siddiqui - Molecular Catalysis, 2020 - Elsevier

9. Compositional Control as the Key for Achieving Highly Efficient OER Electrocatalysis with Cobalt Phosphates Decorated Nanocarbon Florets, J Saha, S Verma, R Ball, C Subramaniam… - Small, 2020 - Wiley Online Library

10. Hybrid silver–gold nanoparticles suppress drug resistant polymicrobial biofilm formation and intracellular infection, E Bhatia, R Banerjee - Journal of Materials Chemistry B, 2020 - pubs.rsc.org

11. Development of oxidation resistant and mechanically robust carbon nanotube reinforced ceramic composites, V Verma, SC Galaveen, L Gurnani… - Ceramics …, 2020 - Elsevier

12. Zeolitic imidazolate framework-67/carboxylated graphene oxide nanosheets incorporated polyethersulfone hollow fiber membranes for removal of toxic heavy …, A Modi, J Bellare - Separation and Purification Technology, 2020 - Elsevier

13. Enhanced wettability and photocatalytic activity of seed layer assisted one dimensional ZnO nanorods synthesized by hydrothermal method, D Upadhaya, DD Purkayastha - Ceramics International, 2020 - Elsevier

14. Di-tert-butylphosphate Derived Thermolabile Calcium Organophosphates: Precursors for Ca(H2PO4)2, Ca(HPO4), α-/β-Ca(PO3)2, and Nanocrystalline Ca10(PO4)6 …, S Verma, R Murugavel - Inorganic Chemistry, 2020 - ACS Publications


1. G. Sridhar, N. Birbilis and V.S. Raja, The reliability of metastable pit sizes estimated from dissolution current in aluminum alloys, Corrosion Science, Volume 182, 15 April 2021, 109276

2. MohdMoiz Khan, S.M. Mahajani, G.N. Jadhav, Transformation of bentonite used in green sand molds during metal casting process and its relevance in sand reclamation, Applied clay science, volume 206, 1 June 2021, 106072

3. Jinal M. Mehta, Nishant K. Jain, Deepak S. Chauhan, Rajendra Prasad, Mukesh K. Kumawat, Mukesh Dhanka, AsifkhanShanavas, Rohit Srivastava, Emissive radiodense stealth plasmonic nanohybrid as X-ray contrast and photo-ablative agent of cancer cells, Materials Today Communications, Volume 27, 2021,https://doi.org/10.1016 /j.mtcomm.2021.102181

4. Mukesh Dhanka, Vaishali Pawar, Deepak S. Chauhan, Nishant Kumar Jain, Prabhuraj R.S., Chaitra Shetty, Mukesh K. Kumawat, Rajendra Prasad, Rohit Srivastava,Synthesis and characterization of an injectable microparticles integrated hydrogel composite biomaterial: In-vivo biocompatibility and inflammatory arthritis treatment, Colloids and Surfaces B: Biointerfaces, Volume 201, 2021, https://doi.org/10.1016/j.colsurfb.2021.111597.

5. Barkha Singh, Rohan Bahadur, MisahRangara, Mayuri N. Gandhi, and Rohit Srivastava*, Influence of Surface States on the Optical and Cellular Property of Thermally Stable Red Emissive Graphitic Carbon Dots, ACS Appl. Bio Mater.2021, 4, 5, 4641–4651


  1. Khandekar Ritesh and Bindu Gopalkrishnan, Pharmacognostic evaluation of Sphagneticola calendulacea (L.) Pruski: Leaves, Journal of Pharmacognosy and Phytochemistry, Vol. 11, Issue 3 (2022)
  2. Shivnetra Rampalli & Bindu Gopal Krishnan, Pharmacognostical investigation of Clitoriaternatea L.Leaves, Plant Science Today, Vol.9, No. 4, 2022,814–819
  3. Shivnetra Rampalli and Bindu Gopalkrishnan, Standardization of Clitoria annua var. Emarginata. S.R.Yadav & Dhanke: Leaves, Journal of Pharmacognosy and Phytochemistry,Vol.11,Issue 2(2022)315-321
  4. Anoop Singh, Asha Sharma and Sandeep Arya, Human sweat-based wearable glucose sensor on cotton fabric for real-time monitoring, Journal of Analytical Science and Technology, 13,11,01 April 2022
  5. R. Goswami and A. K. Dey, Cationic dye removal using surface treated activated carbon as an adsorbent, The Royal Society of Chemistry 2022, 7th September 2022, Pg.22
  6. H. K. Mahilarya and A. K. Dey, Preparation and application of carboxylated and mechanically attrited carbon for adsorptive removal of crystal violet dye, The Royal Society of Chemistry 2023, 2023, 9, 861
  7. Shanmugam Sudarshan, Vidya Shree Bharti, Sekar Harikrishnan, Satya Prakash Shukla, Govindarajan Rathi, Bhuvaneswari, Eco-toxicological efect of a commercial dye Rhodamine B on freshwater microalgae Chlorella vulgaris, Archives of Microbiology (2022) 204:658, (2022) 204:658
  8. Rumi Goswami and Amit Kumar Dey, Use of Anionic Surfactant-Modified Activated Carbon for Efficient Adsorptive Removal of Crystal Violet Dye, Volume 2022, Pg 28
  9. Ipsita Das, Amit Arora, One stage hydrothermal treatment: A green strategy for simultaneous extraction of food hydrocolloid and co-products from sweet lime (Citrus Limetta) peels, Food Hydrocolloids, 2023, 107947.
  10. Neha Redkar, Madhurima Deb, Cathrine Manohar, Ashok Jagtap, Sumit Saxena and Shobha Shukla, Biomimetic Graphene Oxide Architectures as Nanocarriers for Synergistic Methylene Blue Degradation,12th-14th August 2022.
  11. Neha Redkar, Cathrine Manohar, Sumit Saxena, and Shobha Shukla, Effect of Functionalized Graphene Oxide on Bacterial Lipase Activity Tailored for Environmental Application, 20-22nd October 2022.


  1. Ruchira Javkar*, Anil Avhad, Effect of drought and salinity stress on Calcium oxalate crystals ofPortulacaria afra. (L.) Jacq., Journal of Stress Physiology and Biochemistry, Vol.19 No. 1, 2023
  2. Venkata Siva Naga Sai Goli a, Rajendra Yadav b, Manager Rajdeo Singh, Forensic investigations on 1900 years old brick and mortar samples from Buddhist stupa located at Nalasopara, India, Construction and Building Materials, Volume 367, 27 February 2023, 130281
  3. Priyanka Roy, Vijay K. Gahlawat, Chakkaravarthi Saravanan, Bhim P. Singh, Enhancing bioflavor production by solid-state fermentation using Kluyveromyces marxianus and l-phenylalanine, Journal of Basic Microbiology, Volume 63, January 2023, Pg.17
  4. Barkha Singh, Rohan Bahadur, Priyanka Maske, Mayuri Gandhi, Dipty Singh and Rohit Srivastava, Preclinical safety assessment of red emissive gold nanocluster conjugated crumpled MXene nanosheets: a dynamic duo for image-guided photothermal therapy, 2023, 15, 2932.
  5. Barkha Singh, Chetna Patnaik, Rohan Bahadur, Mayuri Gandhi, Abhijit De and Rohit Srivastava, Synthesis and degradation mechanism of renally excretable gold core–shell nanoparticles for combined photothermal and photodynamic therapy, Nanoscale, 2023,15,1273.
Instructions for sample preparation/submission
  • Link for internal registration: https://drona.ircc.iitb.ac.in/ircc/NewFac/CentralFacilityIndex.jsp?facilityCode=RS001
  • Link for external registration: http://www.saif.iitb.ac.in/
  • Medium for Dispersion Ethanol /Methanol / Water /Iso-propyl alcohol. Any other medium should be provided by the user. Dispersion will be done by ultrasonication.
  • Kindly mark the edge of the sample to be observed for Cross section.
  • Base of the sample should be flat for mounting on sample holder.
  • Biological samples will be accepted only after primarily fixation with suitable fixative.
  • Sample preparation if any should be done at user end (cutting the sample for CS, freeze fracturing, sample fixation for biological samples, staining of samples, oven drying should be done by the user)
  • For any further query, kindly contact on Email: esem [at] iitb [dot] ac [dot] in, Contact: 022 21596862
Instructions for Users
Instructions for Registration