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- Bruker 5 mm SmartProbeTM (standard room-temperature BBO probe) for multinuclear measurements of small molecules. The probe can be tuned to observe 19F or any nucleus in the range from 31P- 199Hg and 17O-109Ag excluding 171Yb-9Be. Simultaneous pulsing on 1H and 19F nuclei is possible, enabling the recording of 19F spectra with 1H decoupling. The probe is equipped with a z-gradient (5 G/A*cm), 2H locking and is capable of carrying out solvent suppression using pre-saturation or pulsed-field gradients.
- 5mm Triple resonance (TXI) high-resolution room temperature-probe (with activelyshielded 5 G/A*cm z-gradient and 2H lock). The probe can be used for structural and dynamic analysis of doubly (13C/15N) or singly (15N) labelled biomolecules, using 1H detected measurements and 13C/15N decoupling.
Probe specifications
- Bruker 5 mm SmartProbeTM (standard room-temperature BBO probe) for multinuclear measurements of small molecules. The probe can be tuned to observe 19F or any nucleus in the range from 31P- 199Hg and 17O-109Ag excluding 171Yb-9Be. Simultaneous pulsing on 1H and 19F nuclei is possible, enabling the recording of 19F spectra with 1H decoupling. The probe is equipped with a z-gradient (5 G/A*cm), 2H locking and is capable of carrying out solvent suppression using pre-saturation or pulsed-field gradients.
- 5mm Triple resonance (TXI) high-resolution room temperature-probe (with actively-shielded 5 G/A*cm z-gradient and 2H lock). The probe can be used for structural and dynamic analysis of doubly (13C/15N) or singly (15N) labelled biomolecules, using 1H detected measurements and 13C/15N decoupling.
NMR spectroscopy is a high resolution analytical method for structure, conformational analysis and dynamics of molecules. NMR-active nuclear spins are excited by the application of radiofrequency pulses of matching frequency. The magnetic field generated by these spins is detected by the same RF coil which is used to excite them, giving rise to the NMR signal (FID). Signal processing results in a frequency domain spectrum which is then analyzed to reveal information about the environment around the spins.
Inorganic/organic small molecule samples which are highly soluble (minimum concentration 1 mM- 2mM) in typical NMR solvents (deuterated methanol, deuterated DMSO, deuterated acetone, deuterated chloroform or D2O etc.) can be analyzed. The molecular weight and solubility must be clearly stated when sending in samples.
Isotopically labelled samples (15N labelled or 13C/15N labelled samples) can be sent in lyophilized form or on ice in the relevant buffer. If sent in lyophilized form, please include the NMR sample buffer. Sample concentrations should be 500 μM or more for 2D experiments and 1 mM or more for 3D measurements. Currently, long experiments are possible only at room temperature, depending upon the stability and solubility of the biomolecular samples.