Nuclear Magnetic Resonance Spectrometer- 600 MHz
Description
This facility enables the recording and analysis of a number of conventional 1D and 2D NMR experiments for liquid organic/inorganic small molecule samples as well as 3D measurements for protonated and isotopically labelled biomolecules, below ~15 kilodaltons. These experiments are vital for identification, assignment and structural/dynamic characterization of a wide range of molecules.
Make and Model
Bruker 600 MHz, Avance Neo Console
Available mode for use
iProbe; TXI probe (solution)
Specifications/Features
- Bruker 5 mm SmartProbeTM (standard room-temperature BBO probe) for multinuclear measurements of small molecules. The probe can be tuned to observe 19F or any nucleus in the range from 31P- 199Hg and 17O-109Ag excluding 171Yb-9Be. Simultaneous pulsing on 1H and 19F nuclei is possible, enabling the recording of 19F spectra with 1H decoupling. The probe is equipped with a z-gradient (5 G/A*cm), 2H locking and is capable of carrying out solvent suppression using pre-saturation or pulsed-field gradients.
- 5mm Triple resonance (TXI) high-resolution room temperature-probe (with actively-shielded 5 G/A*cm z-gradient and 2H lock). The probe can be used for structural and dynamic analysis of doubly (13C/15N) or singly (15N) labelled biomolecules, using 1H detected measurements and 13C/15N decoupling.
Application
Small Molecules
- 1D 1H: with and without solvent suppression
- 1D NOESY, 1D DOSY, 1D CPMG (for T2 measurement), 1D Inversion Recovery (for T1 measurement)
- 1D 13C, 1D 19F, 1D 31P (coupled and decoupled versions), DEPT90, DEPT135
- 2D Experiments: NOESY, TOCSY, COSY, DQF-COSY, HETCOR, HSQC, HMBC
- 1D experiments on other X-nuclei (direct detection) - available upon request
Biomolecules
- Standard 15N-1H HSQC, 13C-1H HSQC experiments
- 3D Experiments for protein backbone and sidechain assignments:
- HNCO, HNCA, HN(CO)CA, HN(CA)CO
- HNCACB, CBCACONH, CC(CO)NH
- TOCSY-HSQC, etc.
- 3D 15N/13C edited NOESY-HSQC experiments for distance restraints
- Dynamics: Backbone (15N) R1, R2, hetNOE experiments
Variable Temperature Measurements
For low-temperature measurements, a liquid nitrogen exchanger is currently available. Liquid nitrogen charges will be extra. The user needs to inform at least 10 days in advance if they plan to carry out VT experiments. The details must be discussed before proceeding, depending on the current status.
Faculty in-charge
Contact Email
nmr600ioe@chem.iitb.ac.in
Location
Room 117, Ground Floor, CRNTS Building I.I.T. Bombay, Powai, Mumbai - 400076
Contact No: 022-2576- 6687
Other Contact Person
- Dr. Ipsita Chakraborty