The present invention relates to a method for non-destructive yes / no type sensor colorimetric detection of carbamate and organophosphorus pesticides from a surface involving an enzyme coated paper and a substrate paper.
Figure (1) Change in color in the sensor due to presence of 500 ppb and 50 ppb of pesticide (chlorpyrifos) eggplant surface; (2) Change in color in the sensor due to presence of 500 ppb and 50 ppb of chlorpyrifos
In many countries, agricultural exports face border rejections due to pesticide contamination exceeding Maximum Residual Limits (MRLs). The agricultural sector, especially small farmers, lacks a simple, cost-effective tool to detect pesticide levels on their produce. Existing detection methods are often expensive, complex, and require specialized equipment, limiting their accessibility for daily use in the field.
- Non-Destructive, Colorimetric Detection: The technology offers a simple, non-destructive way to detect pesticide residues on agricultural produce using a Yes/No color change (white for unsafe, blue for safe).
- No Need for Sophisticated Equipment: Unlike other methods that require expensive laboratory instruments, this sensor uses enzyme-coated paper and substrate paper for a fast and straightforward field test.
- Customizable for Various MRLs: The sensor can be tailored to detect pesticides according to the MRL levels set by different countries or testing agencies, making it adaptable for international standards.
- Eco-Friendly and Cost-Effective: The sensor is biodegradable, uses non-hazardous chemicals, and has a low manufacturing cost, making it accessible to small farmers.
- Quick Results: Provides results within 5 minutes, allowing for immediate on-site pesticide level verification without delays or complex procedures.
The prototype consists of a paper-based sensor featuring enzyme-coated paper with acetylcholinesterase and substrate paper coated with Indoxyl acetate. To detect pesticides, a swabbing solution containing Isopropyl Alcohol (IPA) and TRIS buffer is applied to the surface of the produce. The user swabs the surface, incubates the enzyme-coated paper, and observes a color change from white to blue, indicating whether the pesticide level is below or above the Maximum Residual Limit (MRL). The enzyme is activated by a buffer solution to trigger a colorimetric reaction. This simple, easy-to-use, field-deployable sensor requires no specialized equipment or additional instrumentation, making it ideal for on-site pesticide testing.
The technology is at hypothesis testing, demonstration in lab stage.
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This technology improves public health by quickly detecting pesticide residues, ensuring food safety and reducing chemical exposure. It empowers small farmers with an affordable tool to monitor pesticide use, helping them comply with international standards. By reducing border rejections, it boosts agricultural exports, increasing farmers' incomes and supporting the national economy. Additionally, the biodegradable sensor promotes sustainability and environmentally friendly farming practices.
- Agriculture: Direct application for farmers, agricultural exporters, and quality assurance in pesticide management
- Food Safety: Ensures that food products meet international safety standards, reducing the risk of contamination
- Environmental Sustainability: Supports sustainable agricultural practices by preventing the overuse of pesticides and enabling traceability in food production
- Export Compliance: Useful for agricultural producers in developing countries, ensuring compliance with international pesticide standards and reducing border rejections
- Healthcare: Contributes to public health by ensuring that pesticide residues in food are below harmful levels
Geography of IP
Type of IP
202021024457
459746