The current diagnostic methods for the amplification of nucleic acid are expensive, require multiple steps, and are not suitable for point-of-care settings, particularly in resource-limited environments. The existing methods typically involve complex processes like PCR that require costly equipment and multiple temperature cycling that makes the entire processes time consuming and need expert operators to perform the test.
This invention provides a method and a device for the single-step disinfection, thermal lysis and isothermal amplification of nucleic acid from the target cell (particularly targeting Mycobacteria). It simplifies the detection process, making it highly efficient and suitable for point-of-care diagnostics by integrating bot lysis and DNA amplification into one step at a consistent temperature of 65°C.
- Cost Effectiveness: Eliminates the need for expensive equipment like thermocyclers due to the involvement of
- Single-Step Process: Combines thermal lysis and isothermal amplification into a single reaction step
- Constant Temperature: Operates effectively at 65°C utilizing helicase-dependent amplification thus eliminating the need for a thermocycler
- Simplicity: Eliminates the need for multiple steps and manual intervention, reducing potential errors and procedural complexity
- No Washing or Purification Needed: Simplifies the process by working effectively in the presence of cell debris
- Accessibility: Suitable for point-in-care applications, enabling rapid, on-site diagnostic capabilities
- Efficiency: Streamlines the process by removing the need for disinfection, cell washing or DNA purification, maintaining high sensitivity and specificity
Proof-of-concept demonstrated.
At present a proof-of-concept has been demonstrated in the lab environment with cultured bacterial samples
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- Improved Healthcare Accessibility: Makes the diagnosis accessible in remote areas.
- Reduced Healthcare Costs: Lowers the costs of diagnostics significantly, allowing for more widespread testing.
- Enhanced Disease Management: Faster diagnosis leads to quicker treatment initiation, potentially reducing transmission rates
Healthcare and Diagnostics, Biomedical research, Forensic Science, Agriculture
- Medical Diagnostics: Particularly useful for rapid tuberculosis testing at the point-of-care, including remote or resource-limited settings.
- Public Health: Enables quick screening in epidemic situations and in regions of high incidence of disease-causing bacterium.
- Research: Facilitates studies on various types of cells by simplifying sample preparation
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