This invention shows the growth and degradation capabilities of P. aeruginosa and E. aurantiacum on hazardous organic compounds (HOCs). Both bacteria showed significant growth and degradation abilities on various HOCs, with preservation techniques using paper discs and corncob matrices demonstrating successful long-term storage of bacterial cells at room temperature for up to six months. Growth and degradation assays after preservation confirmed the retained activity of both free and immobilized bacterial cells on pyrene. Overall, the study highlights the potential of paper and corncob matrices for cost-effective bacterial preservation without refrigeration, offering promising applications in bioremediation and beyond.
Type of IP
Faculty
Category of Patent
Pollution reduction through effective bioremediation of hazardous organic compounds contaminating soil and water.
Cost Savings: Eliminates the need for expensive refrigeration equipment.
Accessibility: Low-cost, easy implementation benefits areas lacking infrastructure.
Sustainability: Uses natural, locally available materials, reducing reliance on synthetic chemicals.
- Acclimatization and Substrate Specificity: HOC degrading bacteria are acclimatized on a sterile mineral medium with substrates and specific HOCs like phenol, o-cresol, 1-naphthol, quinoline, naphthalene, pyridine, and pyrene, ensuring optimal adaptation and performance.
- Immobilization on Diverse Carriers: Bacteria are immobilized on carriers such as paper discs or corncob, providing stability and protection. This method uses widely available, low-cost materials, enhancing practicality and scalability.
- Protective Agents for Enhanced Stability: Incorporates protective agents to preserve bacterial viability, allowing long-term storage at ambient temperatures.
- Innovative Carrier Preparation: Corncob carriers require washing, drying, grinding, and chemical treatment for producing a fine powder (0.5–1 mm), for ensuring effective immobilization and protection of bacteria. The paper disc carriers require coating at least one side of the discs with a protective agent at a slightly elevated temperature (40-45 °C) followed by drying.
- Inoculation and Storage Process: The paper discs coated with protective agents, are inoculated with bacterial suspension, dried, and stored. Similarly, the pretreated corncob powder is mixed with bacterial suspension, dried and stored. The inoculated paper discs and corncob powder prepared in this manner enable simple and efficient preservation of bacteria at room temperature.
- Acclimatization and Substrate Specificity: HOC degrading bacteria are acclimatized on a sterile mineral medium with substrates and specific HOCs like phenol, o-cresol, 1-naphthol, quinoline, naphthalene, pyridine, and pyrene, ensuring optimal adaptation and performance.
- Immobilization on Diverse Carriers: Bacteria are immobilized on carriers such as paper discs or corncob, providing stability and protection. This method uses widely available, low-cost materials, enhancing practicality and scalability.
- Protective Agents for Enhanced Stability: Incorporates protective agents to preserve bacterial viability, allowing long-term storage at ambient temperatures.
- Innovative Carrier Preparation: Corncob carriers require washing, drying, grinding, and chemical treatment for producing a fine powder (0.5–1 mm), for ensuring effective immobilization and protection of bacteria. The paper disc carriers require coating at least one side of the discs with a protective agent at a slightly elevated temperature (40-45 °C) followed by drying.
- Inoculation and Storage Process: The paper discs coated with protective agents, are inoculated with bacterial suspension, dried, and stored. Similarly, the pretreated corncob powder is mixed with bacterial suspension, dried and stored. The inoculated paper discs and corncob powder prepared in this manner enable simple and efficient preservation of bacteria at room temperature.
4
TRL-level: TRL-4
Current Status of Technology: Available for technology transfer
Prototype details: NA
Biotechnology, Waste Management, Industrial wastewater treatment
Bioremediation: Treatment of hazardous waste oil spills and pollutants in various environments.
Agriculture: Remediation of soil and water contaminated by agricultural chemicals and livestock waste.
Water Resource Management: Purification of drinking water and treatment of aquaculture effluents.
Urban Development: Cleanup of contaminated sites for urban renewal projects. Waste Management: Treatment of landfill leachate and organic waste streams.