Membrane protein purification using engineered micelles


■Membrane proteins comprise one third of the transcription products of human genome                                                                                                                                  ■Associated with diverse human diseases and key target for drug development                                                                                                                                            ■X-ray crystal structures of membrane proteins are scarce due to paucity of good quality crystals                                                                                                                  ■Developed a new crystallisation medium that relies on conjugation of micelles with hydrophobic chelator-metal complexes or hydrophobic nucleosides                            ■ Chelator-metal complexes or nucleoside base pairs formed at the interphase facilitate nucleation and crystallisation of membrane proteins                                              ■ Simple methodology that can precipitate membrane proteins in their native environment                                                                                                                              ■ May be applicable to a diverse array of membrane proteins