Laser scanning confocal facility @BSBE
Description
Laser scanning confocal microscopy employs spatial filtering techniques to eliminate any out-of-focus light in specimens with finite thickness and leads to the formation of high-resolution images.
Make and Model
Carl Zeiss, LSM 780
Available mode for use
Single colour and two-colour imaging and brightfield imaging Z-stacks Time series (with or without Z-stack)
Specifications/Features
- Travel range of 250 μm.
- Objectives: Plan-apochromat 10X/0.45 NA(air), 20X/0.8 NA (air), C-apochromat 40x/1.2 NA (water) for FCS measurements, Plan-Apochromat 40x/1.3 NA (oil) iPlan-apochromat 63x/1.4 NA (oil), iPlan-apochromat 100x/1.4 (oil). DIC imaging is possible with the last three objectives.
- Lasers: Ar+laser, DPSS laser and HeNe laser, Titanium sapphire multiphoton laser
- Zen 2012 acquisition software
- Temperature and CO2 -controlled incubation stage for long term live cell imaging.
- Lifetime imaging through a FLIM attachment
- Non de-scanned detector available for use with the multiphoton laser for deep tissue imaging.
Application
- Multi-colour imaging
- Z- stacks and 3D image reconstruction
- Time series (with or without Z-stack)
- Tile scanning to image different parts of the sample automatically over long durations)
- FRET, FRAP, FCS/FCCS
- FLIM
Faculty in-charge
Contact Email
lsmconfocal@iitb.ac.in
Location
Central Instrumentation Room, Ground floor Biosciences & Bioengineering Department. I.I.T. Bombay, Powai, Mumbai - 400 076.
Contact No: 022-2159 6746
Other Contact Person
- Pradip Shinde
- Santosh Panigrahi